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We recently reported that iPSCs can
2018-10-20
We recently reported that iPSCs can be generated using non-modified mRNA (Arnold et al., 2012). In this report we will focus on the crucial steps for successfully applying this method. Material and methods Results Discussion Acknowledgments The work was supported by funding from the Ger
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br Materials and methods br
2018-10-20
Materials and methods Results and discussion To generate an OKSM mouse model with higher reprogramming efficiency we exchanged the m2rtTA for the more efficient rtTA3 (Das et al., 2004). We used founder animals which can be found commercially available at the Jackson Institute and applied a br
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The transgenic iPSCs differentiate into embryoid
2018-10-20
The transgenic iPSCs differentiate into embryoid bodies (EBs) containing spontaneously beating EGFP-positive areas, which first appear on day 7–9 of differentiation (Fig. 2A). After 7days of puromycin treatment, nearly all non-cardiomyocytes in EBs are eliminated and the resistant h2 agonist surviv
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The dedifferentiation of lineage committed cells
2018-10-20
The dedifferentiation of lineage-committed Cell Cycle Compound Library into more primitive stem-like states during transplantation is similar to the cell-state transitions that occur during tumor initiation (Keller et al., 2010). For example, preceding the onset of basal cell carcinoma, adult tumor-
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Whole exome sequencing of tumor
2018-10-20
Whole-exome sequencing of tumor DNA from GBM 5 identified a total of 32 SNVs (Table S3). A subset of these SNVs was selected for single-cell analysis based on putative gene function and variant allele fractions encompassing high, low, and intermediate frequencies. The genomic targets selected includ
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Human leukocyte class I HLA I molecules are
2018-10-20
Human leukocyte class I (HLA-I) molecules are critical for antigen presentation to CD8+ cytotoxic T-lymphocyte (Garrido et al., 1993), and are understood to be widely expressed on the cell surface of all normal human tissues (Daar et al., 1984). However, HLA-I expression has been reported to be abse
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br Experimental Procedures br Author Contributions br Acknow
2018-10-20
Experimental Procedures Author Contributions Acknowledgments The research leading to these results has received funding from the European Community’s Seventh Framework Programme under grant agreement n.602300 (SYBIL) to A.V., PRIN Project 20102M7T8X_003 to A.V., Ricerca Finalizzata from Min
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Analysis of TCR signatures predicts
2018-10-20
Analysis of TCRβ signatures predicts that several initial “founder” clones of iPSC were present at P4 but that these resolved to a single clone by P20. This was somewhat surprising, as it is often assumed that individually picked colonies of iPSC are derived from a single cell and are therefore clon
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Finally an important practical implication of our data is
2018-10-20
Finally, an important practical implication of our data is that both fibroblasts and blood can be used for the generation of comparable iPSC lines for large-scale biobanking purposes (Figure 6). Our results demonstrate that variability among small cohorts of iPSCs may lead to erroneous conclusions (
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Main Text br Acknowledgments I have
2018-10-20
Main Text Acknowledgments I have been fortunate to work with tremendous mentors, colleagues, and students throughout my career and I am grateful for their past and current support. Research in my laboratory is supported by the NIH, the New York Stem Cell Foundation as a New York Stem Cell Founda
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br Results br Discussion The Wnt signaling
2018-10-20
Results Discussion The Wnt signaling pathway has emerged as the dominant self-renewal pathway for various adult-type stem cells and is required for maintenance of embryonic as well as induced pluripotent stem cells. In the hematopoietic system, only mild increased Wnt dosages result in higher
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br Experimental Procedures br Author
2018-10-20
Experimental Procedures Author Contributions Acknowledgments We wish to thank the cytometer unit of CABIMER for technical support, Diana Aguilar for critical reading of the manuscript, and Veronica Raker for style corrections. This work was funded by an R + D + I grant from the Spanish Mini
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Hello world!
2018-07-29
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